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rabbit anti cx40 19  (Alpha Diagnostics)

 
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    Structured Review

    Alpha Diagnostics rabbit anti cx40 19
    Rabbit Anti Cx40 19, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-cx40/anti+eaac1+eaat3+polyclonal+primary+rabbit/pm42252105-104-32-35
    Average 86 stars, based on 1 article reviews
    rabbit anti cx40 19 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).

    Western Blot:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).

    In Vitro:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).

    Cell Culture:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).

    Transplantation Assay:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).

    Generated:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).

    Immunofluorescence:

    Article Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Article Snippet: The following primary antibodies were used [species, target, dilution, company, and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); goat anti-Cntn2, 1:100 for immunofluorescence (IF) staining and 1:1000 for WB analysis (R&D Systems); mouse mAb α-Tubulin (DM1A), 1:1000 for WB analysis (Cell-Signaling Technology, #3873); rabbit polyclonal anti-Hcn4, 1:200 for IF staining and WB analysis (Alomone Labs, #APC-052); mouse anti-sarcomeric actinin, 1:200 (Sigma); and Rabbit anti-Myl2 Polyclonal antibody, 1:100 (ProteinTech).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.
    Article Snippet: The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration.. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells.. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion.

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 μg/ml for IF staining and 1 μg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 μg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262).

    Article Title: Lack of connexin 40 decreases the calcium sensitivity of renin secreting juxtaglomerular cells
    Article Snippet: Sagittal sections (5 μm) were blocked with 10 % horse serum/1 % BSA in PBS before the primary antibodies (chicken anti-renin: Davids Biotech.Regensburg,Germany, rabbit anti-Cx40: Alpha Diagnostic Intl.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
    Article Snippet: The following primary antibodies were used: rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); rat anti-CD31 (BD Biosciences; 557355); mouse anti-Sall1 (Perseus Proteomics; PP-K9814-00); rat anti-CK8 (Developmental Studies Hybridoma Bank, University of Iowa; Troma-I); goat anti-NRP2 (R&D Systems; AF567); chick anti-GFP (Abcam; ab13970); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-Six2 (Proteintech; 11562-1-AP); and guinea pig anti-Nephrin (Progen Biotechnik; GP-N2).

    Article Title: Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1.
    Article Snippet: We used rat anti-PECAM-1 (clone Mec13.3, BD PharMingen), fluorescein isothiocyanate (FITC)-conjugated mouse anti-a-smooth muscle actin (clone 1A4, Sigma), goat anti-Nrp2 (R&D Systems), goat anti-VEGFR3 (R&D), rabbit anti-Cx40 (Alpha Diagnostics), rat anti-MECA32 (BD PharMingen), goat antiPlexinD1 (R&D), Cy3-conjugated mouse anti-FLAG (clone M2, Sigma), and mouse anti-b-actin (Sigma) antibodies.

    Article Title: Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Article Snippet: The following primary antibodies were used: chick anti-GFP (Abcam; ab13970); rabbit anti-Cx40 (Alpha Diagnostic; CX40-A); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-CD31 (Abcam; ab28364); rat anti-CD31 (BD Biosciences; 557355); rabbit anti-Six2 (Proteintech; 11562-1-AP); rabbit anti-red fluorescent protein (RFP) (Rockland; 600-401-379); rat anti-TER-119 (MBL; D062-3); and guinea pig anti-nephrin (Progen Biotechnik; GP-N2).



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    Image Search Results


    Distribution of Cx37 within the internal elastic lamina. A . Representative 2-photon-image of a small resistance artery for visualization of the internal elastic lamina and the connexins. Cx37 (red) located in the small holes (dark dots) within the internal elastic lamina (autofluorescence, green). Arrows indicate some of the holes in which Cx37 could be detected; scale bar: 25 μm. B depicts the summary of n = 7-10 experiments (3 vessels each) revealing the percentage of holes in the internal elastic lamina that contain the different vascular Cx (*: p < 0.05 vs. Cx40; #: p < 0.05 vs. Cx45, NG).

    Journal: Cell Communication and Signaling : CCS

    Article Title: NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions

    doi: 10.1186/1478-811X-12-33

    Figure Lengend Snippet: Distribution of Cx37 within the internal elastic lamina. A . Representative 2-photon-image of a small resistance artery for visualization of the internal elastic lamina and the connexins. Cx37 (red) located in the small holes (dark dots) within the internal elastic lamina (autofluorescence, green). Arrows indicate some of the holes in which Cx37 could be detected; scale bar: 25 μm. B depicts the summary of n = 7-10 experiments (3 vessels each) revealing the percentage of holes in the internal elastic lamina that contain the different vascular Cx (*: p < 0.05 vs. Cx40; #: p < 0.05 vs. Cx45, NG).

    Article Snippet: After washing twice, and perfusing the for 5 min with PBS the vessels were incubated and perfused for 2 hours with PBS containing 1% BSA and 0.3% Triton-X100, washed and perfused again with PBS-1% BSA and incubated overnight with the antibodies against Cx37 (10 μg/ml Alpha Diagnostics, Cat. No. Cx37A11-A), Cx43 (see above), Cx40 (3.3 μg/ml polyclonal rabbit anti mouse-Cx40, Alpha-Diagnostics, Cat. No. Cx40-A) or Cx45 (2.5 μg/ml polyclonal rabbit anti Cx45 c-Term, Zymed, Cat. No. 40–7000) and against CD31 (2.5 μg/ml monoclonal rat anti mouse-CD31, Abcam, Cat. No 56299; antibodies were applied from the endothelial and smooth muscle side).

    Techniques:

    Location of vascular Cx across the vessel wall. A . Confocal images of triple (Cx, α-actin, CD31) immunohistochemical stainings of Cx40, Cx45, Cx37, and Cx43 in small resistance arteries. Left panel: Overlay of a z-series in xy-direction, right panel: Cross section (slice) of the z-stack in yz-direction along the yellow lines in the z-stack. The arrows depict the Cx expression in ECL (yellow) or beyond EC and within SMC (SMCL, white), scale bars: 10 μm. B . Summary of the Cx distribution within the ECL and SMCL for all Cx (n = 4-8, at least 3 vessels each; *: p < 0.001).

    Journal: Cell Communication and Signaling : CCS

    Article Title: NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions

    doi: 10.1186/1478-811X-12-33

    Figure Lengend Snippet: Location of vascular Cx across the vessel wall. A . Confocal images of triple (Cx, α-actin, CD31) immunohistochemical stainings of Cx40, Cx45, Cx37, and Cx43 in small resistance arteries. Left panel: Overlay of a z-series in xy-direction, right panel: Cross section (slice) of the z-stack in yz-direction along the yellow lines in the z-stack. The arrows depict the Cx expression in ECL (yellow) or beyond EC and within SMC (SMCL, white), scale bars: 10 μm. B . Summary of the Cx distribution within the ECL and SMCL for all Cx (n = 4-8, at least 3 vessels each; *: p < 0.001).

    Article Snippet: After washing twice, and perfusing the for 5 min with PBS the vessels were incubated and perfused for 2 hours with PBS containing 1% BSA and 0.3% Triton-X100, washed and perfused again with PBS-1% BSA and incubated overnight with the antibodies against Cx37 (10 μg/ml Alpha Diagnostics, Cat. No. Cx37A11-A), Cx43 (see above), Cx40 (3.3 μg/ml polyclonal rabbit anti mouse-Cx40, Alpha-Diagnostics, Cat. No. Cx40-A) or Cx45 (2.5 μg/ml polyclonal rabbit anti Cx45 c-Term, Zymed, Cat. No. 40–7000) and against CD31 (2.5 μg/ml monoclonal rat anti mouse-CD31, Abcam, Cat. No 56299; antibodies were applied from the endothelial and smooth muscle side).

    Techniques: Immunohistochemical staining, Expressing